《植物生理学报》 2010, 46(6): 589-596

狗蔷薇类原球茎发育过程的显微观察

姜福星, 田传卫, 张建甫, 马男, 张常青, 赵梁军*

中国农业大学农学与生物技术学院观赏园艺与园林系, 北京100198

通信作者：赵梁军；E-mail: zhaolj5073@sina.com；Tel: 010-62733 315

摘　要：

对狗蔷薇类原球茎发育进行显微观察的结果表明: 类原球茎发育过程中外部形态发生了明显的变化; 毛状不定根及 其产生的类原球茎均具备根的结构特点; 类原球茎由毛状不定根中维管柱顶端外围的中柱鞘薄壁细胞发育而来, 是包括胚 性细胞和薄壁细胞在内的囊腔状结构的复合组织, 胚性细胞在类原球茎中的发育经历了原胚、球形胚、心形胚和鱼雷胚阶 段, 并且与周围的细胞存在明显的生理隔离; 类原球茎具有明显的极性, 并且可以重复发生形成次类原球茎。

关键词：狗蔷薇; 毛状不定根; 类原球茎; 发育过程; 显微观察

收稿：2010-04-16   修定：2010-05-28

资助：国家自然科学基金(30871733)和国家“ 十一五” 支撑计划项目(2006BAD07B01)。

Microscopic Observations on Development of Protocorm-Like Body of Rosa canina L.

JIANG Fu-Xing, TIAN Chuan-Wei, ZHANG Jian-Fu, MA Nan, ZHANG Chang-Qing, ZHAO Liang-Jun*

Department of Ornamental Horticulture and Landscape Architecture, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100198, China

Corresponding author: ZHAO Liang-Jun; E-mail: zhaolj5073@sina.com; Tel: 010-62733 315

Abstract:

In this work, we make a microscopic observation on the development of protocorm-like body (PLB) of dog rose (Rosa canina). Our results indicate that there are obvious external morphological changes during the developing process of PLB, hair adventitious root and PLB which is derived from it have anatomic structure characteristics of the root. PLB originated from the outside pericycle parenchymatous cells of the vascular cylinder tip in hair adventitious root, while it was a complex tissue of pouch cavity shape structure that contained embryonic cell and parenchymatous cell. We found that the development of embryonic cell in PLB underwent classic phases of embryo development including proembryo, globular embryo, heart-shaped embryo and torpedo embryo, and embryonic cell forms obvious physiological isolation from surrounding cells. Furthermore, the PLB has obvious polarity and can form secondary PLB repeatedly.

Key words: dog rose; hair adventitious root; protocorm-like body; development; microscopic observations